Reporter

Part:BBa_K786030:Design

Designed by: ChunHungKong, KenHo, LeonardoKan, Kaiyin Feng   Group: iGEM12_Hong_Kong-CUHK   (2012-09-24)


T7-repeat-spacer-repeat-T7terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 134
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The repeat-spacer-repeat region design should be consistent with the working principles of E.coli's CRISPR/cas system.


Source

The 'repeat' sequence in CRISPR/cas system of E.coli strain K12.

References